Active Recombinant Human PDGFA Protein (Ser87-Thr211), GMP Grade, Animal-Free

• Cat.No.: PDGFA-17HG
• Product Overview: GMP recombinant human PDGF-AA protein (Ser87-Thr211) without tag was expressed in E. coli, produced in an Animal-Free laboratory and manufactured and tested under cGMP guidelines.

Specification

• Species: Human
• Source: E.coli
• Protein Length: Ser87-Thr211
• Description: This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.
• Form: Disulfide-linked homodimer
• Bio-activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is typically 50-200 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin.
• Molecular Mass: Predicted Molecular Mass: 14 kDa
  SDS-PAGE: 15-20 kDa, reducing conditions
• N-terminal Sequence Analysis: Ser87-Ile-Glu-Glu-Ala-Val-Pro-Ala-Val-(Cys)
• Endotoxin: <0.01 EU/ μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining, under reducing conditions.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Reconstitution: Reconstitute at 100-200 μg/mL in sterile 4 mM HCl.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: PDGFA platelet-derived growth factor alpha polypeptide [ Homo sapiens ]
• Official Symbol: PDGFA
• Synonyms: PDGFA; platelet-derived growth factor alpha polypeptide; platelet-derived growth factor subunit A; PDGF A chain; PDGF A; PDGF1; platelet derived growth factor alpha chain; PDGF-1; PDGF A-chain; PDGF subunit A; platelet-derived growth factor A chain; platelet-derived growth factor alpha chain; platelet-derived growth factor alpha isoform 2 preproprotein; PDGF-A
• Gene ID: 5154
• mRNA Refseq: NM_002607
• Protein Refseq: NP_002598
• MIM: 173430
• UniProt ID: P04085