Active Recombinant Human PDE6B, GST-tagged

• Cat.No.: PDE6B-1530H
• Product Overview: Full-length recombinant human PDE6B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: PDE6B is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and cGMP. PDE6B is a high-affinity cGMP-specific PDE that shows high expression in the eye. Four mutations in the PDE6B gene lead to a degenerative disease of photoreceptors called retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Subretinal injection of mice with retinal degeneration with PDE6B gene showed significant decrease in photoreceptor cell death. A nonsense mutation in the PDE6B gene is also the cause of Rod-cone dysplasia-1 (rcd1) in Irish setters.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 19nmol/min/mg
• Molecular Mass: ~124 kDa
• Protein length: Full length
• Purity: >80%
• Applications: PDE Assay
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cyc
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: PDE6B phosphodiesterase 6B, cGMP-specific, rod, beta [ Homo sapiens ]
• Official Symbol: PDE6B
• Synonyms: PDE6B; phosphodiesterase 6B, cGMP-specific, rod, beta; PDEB; rod cGMP-specific 3,5-cyclic phosphodiesterase subunit beta; congenital stationary night blindness 3; autosomal dominant; CSNB3; rd1; RP40; GMP-PDE beta; rod cGMP-phosphodiesterase beta-subunit; CSNBAD2
• Gene ID: 5158
• mRNA Refseq: NM_000283
• Protein Refseq: NP_000274
• MIM: 180072
• UniProt ID: P35913
• Chromosome Location: 4p16.3
• Pathway: Hemostasis, organism-specific biosystem; Nitric oxide stimulates guanylate cyclase, organism-specific biosystem; Phototransduction, organism-specific biosystem; Phototransduction, conserved biosystem; Platelet homeostasis, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem
• Function: 3,5-cyclic-GMP phosphodiesterase activity; hydrolase activity; metal ion binding; phosphoric diester hydrolase activity