Active Recombinant Human PDCD1LG2, Fc-tagged

• Cat.No.: PDCD1LG2-169H
• Product Overview: Recombinant Human B7-DC /PD-L2 /CD273 Protein, with C-Fc Tag (rhB7-DC / PD-L2 / CD273 Fc Chimera) Leu 20 - Pro 219 (Accession # AAI13679) was produced in human 293 cells (HEK293).

Specification

• Description: Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized rh B7-DC / PD-L2 / CD273 Fc Chimera at 1 μg/mL (100 μl/well) can bind rhPDCD-1 with a linear range of 7.5 - 500 ng/mL.
• Molecular Mass: rhB7-DC / PD-L2 / CD273 Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 49.2 kDa. The predicted N-terminus is Leu 20. DTT-reduced Protein migrates as 60-70 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the rhB7-DC / PD-L2 / CD273 Fc Chimera by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
• Reconstitution: See Certificate of Analysis for reconstitution instructions and specific concentrations.
• Protein length: 20-219 a.a.

Gene Information

• Gene Name: PDCD1LG2 programmed cell death 1 ligand 2 [ Homo sapiens ]
• Official Symbol: PDCD1LG2
• Synonyms: PDCD1LG2; programmed cell death 1 ligand 2; B7 dendritic cell molecule; B7 DC; bA574F11.2; Btdc; CD273; PD L2; PDL2; B7-DC; PD-1 ligand 2; PD-1-ligand 2; PDCD1 ligand 2; butyrophilin B7-DC; programmed death ligand 2; B7DC; PD-L2; PDCD1L2; MGC142238; MGC142240
• Gene ID: 80380
• mRNA Refseq: NM_025239
• Protein Refseq: NP_079515
• MIM: 605723
• UniProt ID: Q9BQ51
• Chromosome Location: 9p24.2
• Pathway: Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; PD-1 signaling, organism-specific biosystem
• Function: molecular_function; protein tyrosine phosphatase activity; receptor activity