Active Recombinant Human PDCD1LG2 protein, His-tagged

• Cat.No.: PDCD1LG2-2174H
• Product Overview: Recombinant Human PDCD1LG2 protein(NP_079515.2)(Met1-Pro219), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Pro219
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human PD-L2 at 2 μg/ml (100 μl/well) can bind human PD-1, the EC50 of human PD-1 is 150-800 ng/mL.
• Molecular Mass: The recombinant human PD-L2 consists of 211 amino acids and predicts a molecular mass of 24 kDa. As a result of glycosylation, the apparent molecular mass of rh PD-L2 is approximately 40-45 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.