Active Recombinant Cynomolgus PDCD1 protein(Met1-Gln167), hFc&Avi-tagged, Biotinylated

• Cat.No.: PDCD1-1003C
• Product Overview: Biotinylated Recombinant Cynomolgus PDCD1 (NP_001271065.1) (Met1-Gln167) was expressed in HEK293 with a C-terminal Fc region of Human IgG1 tag followed by an AVI tag.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: C-hFc&Avi
• Protein length: Met1-Gln167
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Cynomolgus CD274 His at 2 μg/mL (100 μL/well) can bind Cynomolgus PD-1 (hFc & AVI Tag), Biotinylated, the EC50 is 30-180 ng/mL.
• Molecular Mass: The recombinant Cynomolgus PDCD1 consists of 396 amino acids and predicts a molecular mass of 44.5 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.