Active Recombinant Human PDCD1, His tagged

• Cat.No.: PDCD1-822H
• Product Overview: Recombinant Human PDCD1 extracellular domain (Met 1-Gln 167) (NP_005009.2), fused with a C-terminal polyhistidine tag, was produced in Human Cell.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 50mM MOPS, 500mM NaCl, pH 7.0
• Bio-activity: 1. Immobilized human PD-1 at 2 μg/ml (100 μl/well) can bind PD-L1 Protein, Human, Recombinant (ECD, hFc Tag), the EC50 of human B7-H1 is 150-600 ng/mL.
  2. Immobilized human PD-1 at 2 μg/mL (100 μL/well) can bind Anti-PD1(MDX)-IgG4 Antibody (Nivolumab),the EC50 is 8-60 ng/mL.
  3. Labeled biotin to PD-1 Protein, Human, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of PD-1 Protein, Human, Recombinant (His Tag), Biotinylated bound to Keytruda was 0.1 nM.
  4.Captured Anti-PD1 Mab (Human IgG4) on proA Chip can bind PD1 with an affinity constant of 10.03nM as determined in a SPR assay (Biacore T200).
  5.Captured Anti-PD-L1 Mab (Human IgG1) on proA Chip can bind PD-L1 with an affinity constant of 1.381nM as determined in a SPR assay (Biacore T200).
• Molecular Mass: The recombinant mature human PD1 consists of 154 amino acids and has a calculated molecular mass of 17.4 kDa. As a result of glycosylation, human PD1 migrates as an approximately 41.1 kDa protein in SDS-PAGE under reducing conditions.
• Protein length: Met 1-Gln 167
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l
• Gene ID: 5133
• mRNA Refseq: NM_005018
• Protein Refseq: NP_005009
• MIM: 600244
• UniProt ID: Q15116