Active Recombinant Human Oligodendrocyte Myelin Glycoprotein, His-tagged

• Cat.No.: OMG-1893H
• Product Overview: Recombinant Human OMgp encoding OMgp (Met 1- Asn 420) with a carboxyl-terminal his tag was expressed inNSO.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 1-420 a.a.
• Description: Myelin Oligodendrocyte Glycoprotein (MOG) is a glycoprotein believed to be important in the process of myelinization of nerves in the central nervous system (CNS). In humans this protein is encoded by the MOG gene. It is speculated to serve as a necessary "adhesion molecule" to provide structural integrity to the myelin sheath and is known to develop late on the oligodendrocyte.
• Purity: > 90%, as determined by SDS-PAGE and visualized by silver stain.
• Format: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: It is recommended that sterile phosphate-buffered saline be added to the vial to prepare a working stock solution of no less than 100 μg/mL. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Endotoxin Levels: < 1.0 EU per 1 μg of the enzyme as determined by the LAL method.
• Molecular Mass: Based on N-terminal amino acid sequencing, the recombinant human OMgp protein starts at Ile 25 and has a calculated molecular mass of 46 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 100 - 120 kDa protein in SDS-PAGE under reducing conditions.
• Activity: Measured by its ability to inhibit neurite outgrowth of cultured embryonic chick dorsal root ganglia neurons. Immobilized OMgp on nitrocellulose-backed microplate (at a 3 μL droplet containing 200 ng) is sufficient to significantly inhibit neurite outgrowth. All experiments were done under carrier-free conditions.
• Storage: Lyophilized samples are stable for up to six months at -20° C to -70° C. Upon reconstitution, this cytokine, in the presence of a carrier protein, can be stored under sterile conditions at 2 - 8° C for one month or at -20° C to -70° C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: OMG oligodendrocyte myelin glycoprotein [ Homo sapiens ]
• Synonyms: oligodendrocyte myelin glycoprotein; OMGP; OMG; oligodendrocyte-myelin glycoprotein
• Gene ID: 4974
• mRNA Refseq: NM_002544.4
• Protein Refseq: NP_002535.3
• MIM: 164345
• UniProt ID: P23515
• Chromosome Location: 17q11.2
• Pathway: Signalling by NGF
• Function: protein binding