Active Recombinant Human NTRK1, His tagged

• Cat.No.: NTRK1-973H
• Product Overview: Recombinant Human NTRK1 (NP_002520.2) amino acid sequence (Pro 285-Glu 413) corresponding to the Ig-like C2-type 2 domain, fused with a N-terminal polyhistidine tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 50mM Tirs, 200mM NaCl, pH 8.0
• Bio-activity: Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-0.8 μg/ml in the presence of 10 ng/mL of Recombinant human NGF.
• Molecular Mass: The recombinant human NTRK1 Ig-like C2-type 2 domain (aa 285-413) consists of 136 amino acids and has a predicted molecular mass of 15.1 kDa. It migrates as an approximately 16 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Pro 285-Glu 413
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NTRK1 neurotrophic tyrosine kinase, receptor, type 1 [ Homo sapiens ]
• Official Symbol: NTRK1
• Synonyms: NTRK1; neurotrophic tyrosine kinase, receptor, type 1; high affinity nerve growth factor receptor; MTC; TRK; TRKA; gp140trk; Oncogene TRK; tyrosine kinase receptor A; tropomyosin-related kinase A; TRK1-transforming tyrosine kinase protein; TRK1; Trk-A; p140-TrkA; DKFZp781I14186
• Gene ID: 4914
• mRNA Refseq: NM_001007792
• Protein Refseq: NP_001007793
• MIM: 191315
• UniProt ID: P04629