Active Recombinant Human NT5E, Fc-tagged, Biotinylated

• Cat.No.: NT5E-579H
• Product Overview: The recombinant human CD73-Fc fusion is expressed as a 759 amino acid protein consisting of Trp27 - Lys547 region of CD73 (Uniprot Accession #P21589) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

Specification

• Source: Human cells
• Species: Human
• Tag: Fc
• Form: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier and preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule).
• Bio-activity: CD73 hydrolyzes the 5"phosphate group from the substrate adenosine 5"monophosphate (AMP) with a specific activity over 20,000 pmoles/min/μg.
• Molecular Mass: Calculated molecular mass (kDa): 84.4; Estimated by SDS-PAGE under reducing condition (kDa): 85-90
• Endotoxin: <0.1 eu per 1 μg of purified recombinant protein determined by the
• Purity: >95% judged by SDS-PAGE under reducing condition
• Storage: The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Avoid repeated freeze-thaw cycles.
• Protein length: 27-547 a.a.

Gene Information

• Gene Name: NT5E 5-nucleotidase, ecto (CD73) [ Homo sapiens ]
• Official Symbol: NT5E
• Synonyms: NT5E; 5-nucleotidase, ecto (CD73); 5 nucleotidase (CD73) , NT5; 5-nucleotidase; CD73; eN; eNT; 5-NT; ecto-5-nucleotidase; Purine 5-Prime-Nucleotidase; NT; NT5; NTE; E5NT
• Gene ID: 4907
• mRNA Refseq: NM_001204813
• Protein Refseq: NP_001191742
• MIM: 129190
• UniProt ID: P21589
• Chromosome Location: 6q14-q21
• Pathway: HIF-1-alpha transcription factor network, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Nicotinate and nicotinamide metabolism, organism-specific biosystem; Nicotinate and nicotinamide metabolism, conserved biosystem; Purine catabolism, organism-specific biosystem
• Function: 5-nucleotidase activity; ferrous iron binding; hydrolase activity, acting on ester bonds; metal ion binding; nucleotide binding