Active Recombinant Human NRXN3 Protein, Fc-tagged

• Cat.No.: NRXN3-432H
• Product Overview: Recombinant human NRXN3 isoform 2 (NP_620426.2) extracellular domain (Met 1-Thr 357) was fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-357 a.a.
• Predicted N Terminal: Ser 36
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to NRXN3 coated plates (0.8 μg/ml and 100 μl/well), >30 % will adhere specifically after 60 minutes at 37°C.
• Molecular Mass: The secreted recombinant human NRXN3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 563 amino acids and predictes a molecular mass of 61.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh NRXN3/Fc monomer is approximately 75-85 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: NRXN3 neurexin 3 [ Homo sapiens ]
• Official Symbol: NRXN3
• Synonyms: NRXN3; neurexin 3; C14orf60, chromosome 14 open reading frame 60; neurexin-3-beta; KIAA0743; neurexin III; neurexin III-beta; neurexin III-alpha; C14orf60; MGC176711
• Gene ID: 9369
• mRNA Refseq: NM_001105250
• Protein Refseq: NP_001098720
• MIM: 600567
• UniProt ID: Q9HDB5
• Chromosome Location: 14q31
• Pathway: Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem