Active Recombinant Human Neurexin 3

• Cat.No.: NRXN3-014H
• Product Overview: A DNA sequence encoding the human NRXN3 isoform 2 (NP_620426.2) extracellular domain (Met 1-Thr 357) was fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Protein Length: 1-357 a.a.
• Description: NRXN3 is a single-pass type I membrane protein which belongs to the neurexin family. It contains one laminin G-like domain. It is a neuronal cell surface protein that may be involved in cell recognition and cell adhesion. Neurexins are a family of proteins that function in the vertebrate nervous system as cell adhesion molecules and receptors.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Purity: > 95 % as determined by SDS-PAGE
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5x104 cells/well are added to NRXN3 coated plates (0.8 μg/mL and 100 μL/well), approximately 30%-50% will adhere specifically after 60 minutes at 37°C
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C.
• Predicted N terminal: Ser 36
• Molecular Mass: The secreted recombinant human NRXN3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 563 amino acids and predictes a molecular mass of 61.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh NRXN3/Fc monomer is approximately 75-85 kDa due to glycosylation.
• Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: NRXN3 neurexin 3 [ Homo sapiens ]
• Official Symbol: NRXN3
• Synonyms: NRXN3; neurexin 3; C14orf60; KIAA0743; MGC176711; neurexin-3-beta; neurexin III; neurexin III-beta; neurexin III-alpha
• Gene ID: 9369
• mRNA Refseq: NM_138970
• Protein Refseq: NP_620426
• MIM: 600567
• UniProt ID: Q9HDB5
• Chromosome Location: 14q31
• Pathway: Cell adhesion molecules (CAMs)
• Function: metal ion binding; receptor activity