Active Recombinant Human NRP1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: NRP1-8119H
• Product Overview: Biotinylated Recombinant Human NRP1 protein(Phe 22 - Lys 644), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Phe 22 - Lys 644
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human VEGF165, premium grade at 2 μg/mL (100 μL/well) can bind Biotinylated Human Neuropilin-1, His,Avitag with a linear range of 0.5-16 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag)The protein has a calculated MW of 73.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NRP1 neuropilin 1 [ Homo sapiens ]
• Official Symbol: NRP1
• Synonyms: NRP1; neuropilin 1; neuropilin-1; CD304; NRP; VEGF165R; transmembrane receptor; vascular endothelial cell growth factor 165 receptor; NP1; BDCA4; DKFZp781F1414; DKFZp686A03134
• Gene ID: 8829
• mRNA Refseq: NM_001024628
• Protein Refseq: NP_001019799
• MIM: 602069
• UniProt ID: O14786