Recombinant Human NRP1 protein, His-tagged

• Cat.No.: NRP1-803H
• Product Overview: Recombinant Human NRP1 aa. (Tyr646~Asp814 (Accession # O14786)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: Tyr646~Asp814
• Description: This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.8kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: NRP1 neuropilin 1 [ Homo sapiens (human) ]
• Official Symbol: NRP1
• Synonyms: NP1; NRP; BDCA4; CD304; VEGF165R; transmembrane receptor; vascular endothelial cell growth factor 165 receptor; neuropilin-1
• Gene ID: 8829
• mRNA Refseq: NM_001024628.2
• Protein Refseq: NP_001019799.1
• UniProt ID: O14786