Active Recombinant Human NR1H3, His-tagged

• Cat.No.: NR1H3-2504H
• Product Overview: Recombinant human His tagged LXRα is isolated from anE. colistrain that carries the coding sequence of the human LXRα under the control of a T7 promoter. 56 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Liver X receptors (LXRs) are nuclear receptors that regulate the metabolism of cholesterol and bile acids. There are two subtypes of LXRs, LXRα and LXRβ. LXRα is preferentially expressed in liver, small intestine, kidney and spleen. In contrast, LXRβ expression is ubiquitous. The genomic structure and the promoter regions of the two LXR genes contain specific regulatory sites, which suggest that LXRs may have physiological roles in the immune system. Like other nuclear receptors, LXRs heterodimerize with retinoid X receptor (RXR) for function. LXRs are activated by naturally occurring oxysterols and regulate the expression of target genes, including ATP binding cassette transporter 1 (ABC1), ATP binding cassette transporter 8 (ABC8) and cholesterol ester transfer protein (CETP). LXRα is thought to play a major role in the control of cholesterol catabolism by stimulating the expression of cholesterol 7 alpha-hydroxylase (CYP7A1), the rate limiting enzyme of bile acid synthesis.
• Applications: LXR can be used for DNA and protein-protein interactions assays.
• Activity: 1 unit equals 1 nanogram of purified protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay.
• Transcript Variant: This variant represents the longest transcript and encodes the longest isoform (a).
• Quality Control: The purified recombinant protein is greater than 95% homogeneous based on SDS-PAGE analysis.
• Reagents Supplied: 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 0.2 mM EDTA and 1mM DTT.
• Storage Conditions: Store at -80°C.
• Pathways: PPAR signaling pathway; Gene Expression; Metabolism of lipids and lipoproteins

Gene Information

• Gene Name: NR1H3 nuclear receptor subfamily 1, group H, member 3 [ Homo sapiens ]
• Synonyms: NR1H3; nuclear receptor subfamily 1, group H, member 3; LXRA; LXR-a; RLD-1; OTTHUMP00000198038; liver X receptor alpha; liver X receptor, alpha; liver X receptor-alpha; TLS/CHOP; Nuclear receptor subfamily 1 group H member 3; Nuclear orphan receptor LXR-alpha
• Gene ID: 10062
• mRNA Refseq: NM_001130101
• Protein Refseq: NP_001123573
• MIM: 602423
• UniProt ID: Q13133
• Chromosome Location: 11p11.2
• Function: metal ion binding; protein binding; sequence-specific DNA binding; thyroid hormone receptor activity; transcription coactivator activity; zinc ion binding; steroid hormone receptor activity