Active Recombinant Human NOTCH3 protein, His-tagged
Cat.No. : | NOTCH3-948H |
Product Overview : | Recombinant Human NOTCH3 protein(Q9UM47)(Ala1378-Ser1640) is expressed from Expi293 with His tag at the C-terminal. |
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Species : | Human |
Source : | HEK293 |
Tag : | His |
Protein Length : | Ala1378-Ser1640 |
Form : | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human Notch 3, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. |
Molecular Mass : | The protein has a predicted MW of 30.18 kDa. Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on Tris-Bis PAGE result. |
Endotoxin : | Less than 1EU per μg by the LAL method. |
Purity : | > 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC |
Storage : | -20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles. |
Reconstitution : | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Gene Name | NOTCH3 notch 3 [ Homo sapiens ] |
Official Symbol | NOTCH3 |
Synonyms | NOTCH3; notch 3; CADASIL, Notch (Drosophila) homolog 3 , Notch homolog 3 (Drosophila); neurogenic locus notch homolog protein 3; CASIL; Notch homolog 3; CADASIL; |
Gene ID | 4854 |
mRNA Refseq | NM_000435 |
Protein Refseq | NP_000426 |
MIM | 600276 |
UniProt ID | Q9UM47 |
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Not For Human Consumption!
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