Active Recombinant Human NKG2C & CD94 protein, His-Avi-tagged, Biotinylated

• Cat.No.: NKG2C & CD94-0573H
• Product Overview: Biotinylated Recombinant Human NKG2C & CD94 protein(Ile94-Leu231(NKG2C)&Asp57-Ile179(CD94)), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 94-231;57-179 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Biotinylated Human NKG2C&CD94, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind HLA-E*01:03 HLA-A leader3-11 Tetramer-VMAPRTLVL-PE,TS-ME01-1.
• Molecular Mass: Molecular CharacterizationThis protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag)The protein has a calculated MW of 34.3 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ile94-Leu231(NKG2C)&Asp57-Ile179(CD94)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.