Active Recombinant Human Neurexin I, Fc-tagged

• Cat.No.: NRXN1-054H
• Product Overview: Recombinant Human NRXN1protein was expressed in Murine myeloma cell line with the Fc part of humanIgG1 Pro100-Lys330 (C-terminus) and Ala51-Ser363 (N-terminus).

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 51-363 a.a.
• Description: The alpha and betaforms of Neurexins 1-3 are transmembrane neuronal glycoproteins which aretranscribed from each of three NRXN genes that utilize alternate promoters.Like other Neurexins, the extracellular domain (ECD) of NRXN1 contains sixLNS domains interspersed with three EGF-like domains, while that of NRXN1contains only the sixth LNS domain and no EGF-like domains. Mature humanNRXN1 is a 70 kDa glycosylated protein with a 313 amino acid (aa) ECD and a56 aa cytoplasmic domain that contains a motif for binding PDZ scaffoldingproteins.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Ala51
• Molecular Weight: 59.9 kDa
• Activity: Measuredby its binding ability in a functional ELISA. Biotinylated rrNLGN-1v2immobilized on a streptavidincoated plate at 1 μg/mL (100 μL/well) can bindrhNRXN1/Fc Chimera with an apparent KD < 4 nM.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 85-95kDa, reducing conditions
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 500 μg/mL in sterile PBS
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt,-20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: NRXN1 neurexin 1 [ Homosapiens ]
• Official Symbol: NRXN1
• Synonyms: NRXN1; neurexin 1;PTHSL2; SCZD17; Hs.22998; neurexin-1-beta; neurexin I; neurexin I-beta;neurexin I-alpha; neurexin-1-alpha
• Gene ID: 9378
• mRNA Refseq: NM_138735
• Protein Refseq: NP_620072
• MIM: 600565
• UniProt ID: P58400
• Chromosome Location: 2p16.3
• Pathway: Cell adhesionmolecules (CAMs)
• Function: acetylcholinereceptor binding; calcium channel regulator activity; calcium-dependentprotein binding; cell adhesion molecule binding; neuroligin family proteinbinding; protein binding; receptor binding; receptor activity; metal ionbinding