Active Recombinant Human NECTIN4 protein, His-tagged

• Cat.No.: NECTIN4-327H
• Product Overview: Recombinant Human NECTIN4 protein(Gly 32 - Ser 349), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gly 32 - Ser 349
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Human Nectin-4, His Tag at 5 μg/mL (100 μL/well) can bind Human PVRL1, Fc Tag with a linear range of 0.01-0.313 μg/mL. Immobilized Human Nectin-4, His Tag at 1 μg/mL (100 μL/well) can bind Mouse Anti-Nectin-4 Antibody with a linear range of 0.2-2 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 36.0 kDa. The protein migrates as 40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.