Recombinant Human NECTIN4 protein, His & T7-tagged

• Cat.No.: NECTIN4-8049H
• Product Overview: Recombinant Human NECTIN4 aa. (Ala31~Val317 (Accession # Q96NY8)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• ProteinLength: Ala31~Val317
• Description: This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.5kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.5.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: NECTIN4 nectin cell adhesion molecule 4 [ Homo sapiens (human) ]
• Official Symbol: NECTIN4
• Synonyms: LNIR; PRR4; EDSS1; PVRL4; nectin-4; Ig superfamily receptor LNIR; nectin 4; poliovirus receptor-related 4; poliovirus receptor-related protein 4; nectin-4
• Gene ID: 81607
• mRNA Refseq: NM_030916.2
• Protein Refseq: NP_112178.2
• UniProt ID: Q96NY8