Active Recombinant Human NCAM1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: NCAM1-0549H
• Product Overview: Biotinylated Recombinant Human NCAM1 protein(Leu20-Gly718), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 20-718 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 500 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human NCAM-1, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-NCAM-1 Antibody, Human IgG1 with a linear range of 1-5 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 81.0 kDa. The protein migrates as 95-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu20-Gly718
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NCAM1 neural cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: NCAM1
• Synonyms: NCAM1; neural cell adhesion molecule 1; CD56; NCAM; neural cell adhesion molecule, NCAM; antigen recognized by monoclonal 5.1H11; MSK39
• Gene ID: 4684
• mRNA Refseq: NM_000615
• Protein Refseq: NP_000606
• MIM: 116930
• UniProt ID: P13591