Active Recombinant Human NAMPT Protein, His-tagged

• Cat.No.: NAMPT-01H
• Product Overview: Recombinant Human PBEF/Visfatin Protein (Asn2-His491) with an N-terminal Met and 6-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.
• Source: E. coli
• Species: Human
• Tag: His
• Bio-activity: Measured by its ability to induce VEGF secretion by PC‑3 human prostate cancer cells. The ED50 for this effect is 1-5 μg/mL
• Molecular Mass: 56 kDa
• N-terminal Sequence Analysis: Met
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: > 90%, by SDS-PAGE with silver staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, EDTA, TCEP and Tween.
• Reconstitution: Reconstitute at 500 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature.
• Protein length: 2-491 a.a.

Gene Information

• Gene Name: NAMPT nicotinamide phosphoribosyltransferase [ Homo sapiens (human) ]
• Official Symbol: NAMPT
• Synonyms: NAMPT; nicotinamide phosphoribosyltransferase; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik; nicotinamide phosphoribosyltransferase; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony-enhancing factor 1; EC 2.4.2.12
• Gene ID: 10135
• mRNA Refseq: NM_005746
• Protein Refseq: NP_005737
• MIM: 608764
• UniProt ID: P43490