Active Recombinant Human NAMPT, Flag-tgged

• Cat.No.: NAMPT-988H
• Product Overview: Visfatin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 466 amino acids. The total molecular mass is 52.6kDa (calculated). The Visfatin is purified by Flag-affinity chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: Flag
• Description: This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.
• Form: Filtered White lyophilized (freeze-dried) powder. Visfatin was lyophilized with no additives.
• Bio-activity: The activity is determined by its ability to induce IL-6, IL-1 beta and TNF alpha production from human PBMCs at 100ng/ml.
• Molecular Mass: 52.6kDa
• AA Sequence: MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Reconstitution: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 20 mM HCl at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Wait several minutes for full reconstitution and solubility.

Gene Information

• Gene Name: NAMPT nicotinamide phosphoribosyltransferase [ Homo sapiens (human) ]
• Official Symbol: NAMPT
• Synonyms: NAMPT; nicotinamide phosphoribosyltransferase; PBEF; PBEF1; pre-B-cell colony enhancing factor 1; Pre-B cell-enhancing factor; Pre-B-cell colony-enhancing factor 1; NAmPRTase; EC 2.4.2.12; VF; 1110035O14Rik; DKFZp666B131; MGC117256; OTTHUMP00000179150; VISFATIN; Visfatin
• Gene ID: 10135
• mRNA Refseq: NM_005746
• Protein Refseq: NP_005737
• MIM: 608764
• UniProt ID: P43490
• Chromosome Location: 7q22.3
• Pathway: Adipogenesis; Metabolism of vitamins and cofactors; Metabolism of water-soluble vitamins and cofactors; Nicotinamide salvaging; Nicotinate and nicotinamide metabolism; Nicotinate metabolism
• Function: cytokine activity; nicotinamide phosphoribosyltransferase activity; nicotinate phosphoribosyltransferase activity; nicotinate-nucleotide diphosphorylase (carboxylating) activity; transferase activity, transferring glycosyl groups