Active Recombinant Human MSLN, His-tagged

• Cat.No.: MSLN-301H
• Product Overview: Recombinant Human MSLN (Accession # AAH09272) Glu296-Gly580, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Glu296
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA.When Recombinant Human CA125/MUC16 is coated at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Mesothelin that produces 50% of the optimal binding response is found to be approximately 0.5 - 2.5 µg/mL.
• Molecular Mass: Recombinant Human MSLN, His-tagged has a calculated MW of 33 kDa. In SDS-PAGE migrates as 40-45 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Protein length: 296-580 a.a.

Gene Information

• Gene Name: MSLN mesothelin [ Homo sapiens ]
• Official Symbol: MSLN
• Synonyms: MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; SMRP
• Gene ID: 10232
• mRNA Refseq: NM_001177355
• Protein Refseq: NP_001170826
• MIM: 601051
• UniProt ID: Q13421
• Chromosome Location: 16p13.3