Active Recombinant Mouse Msln protein, His-tagged

Description :	Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF. MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation. The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is up-regulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression, in part through the up-regulation of MMP-7. It is co-expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion. A soluble form of Mesothelin, with its GPI anchor intact, is released from tumor cells and binds to MMR/CD206 on macrophages.
Source :	CHO
Species :	Mouse
Tag :	His
Predicted N Terminal :	Asp298
Form :	Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
Bio-activity :	Measured by its binding ability in a functional ELISA. When Recombinant Mouse Mesothelin is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CA125/MUC16 that produces 50% optimal binding response is approximately 0.4-2.4 ng/mL.
Molecular Mass :	Predicted Molecular Mass: 35 kDa; SDS-PAGE: 38-55 kDa, reducing conditions.
Protein length :	Asp298-Ser600
Endotoxin :	<0.10 EU per 1 μg of the protein by the LAL method.
Purity :	>95%, by SDS-PAGE with silver staining
Storage :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution :	Reconstitute at 250 μg/mL in PBS.
Shipping :	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Name	Msln mesothelin [ Mus musculus ]
Official Symbol	Msln
Synonyms	MSLN; mesothelin; megakaryocyte potentiating factor; pre-pro-megakaryocyte-potentiating factor; MPF;
Gene ID	56047
mRNA Refseq	NM_018857
Protein Refseq	NP_061345
UniProt ID	Q61468
Chromosome Location	17; 17 A3.3