Active Recombinant Human MRAS (Q71L) Protein, His-tagged

• Cat.No.: MRAS-703H
• Product Overview: Recombinant Human MRAS protein (NM_012219，Q71L) was expressed in E. coli with His tag.

Specification

• Description: Ras proteins are members of the superfamily of small GTP-binding proteins that function as molecular switches controlling a variety of signaling and transport pathways. The Ras family of GTP-binding proteins consists of the classical Ras (Ha-, K-, and N-), R-Ras, Rap1, Rap2, Ral, Rheb, Rin, Rit, TC21/R-Ras2, and M-Ras/R-Ras3 (M-Ras). Stimulation of Ba/F3-Fms cells with either IL-3 or CSF-1 resulted in efficient activation of both K-Ras 4B and M-Ras. Stimulation of fibroblasts with EGF also led to more efficient activation of K-Ras 4B than of H-Ras or N-Ras, and the activation of M-Ras was very strong. An S27N mutant of M-Ras, like the analogous H-Ras S17N mutant, was a dominant inhibitor of activation of the c-fos promoter by constitutively active Src Y527F, suggesting that M-Ras and p21 Ras shared guanine nucleotide exchange factors and are likely to be activated in parallel. M-Ras (Q71L) is a constitutive active mutant of M-Ras.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 25 mM HEPES pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 μM GDP and 50% glycerol
• Bio-activity: GTPγS binding: >750 mmol/mol.
• Purity: > 95 % (SDS-PAGE)
• Storage: Store at -20 centigrade.

Gene Information

• Gene Name: MRAS muscle RAS oncogene homolog [ Homo sapiens ]
• Official Symbol: MRAS
• Synonyms: M-RAs; RRAS3; R-RAS3
• Gene ID: 22808
• mRNA Refseq: NM_001085049.1
• Protein Refseq: NP_001078518.1
• MIM: 608435
• UniProt ID: O14807