Active Recombinant Human MPL protein, His-tagged

• Cat.No.: MPL-1935H
• Product Overview: Recombinant Human MPL protein(Gln 26 - Trp 491), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Human Thrombopoietin R, His Tag captured on CM5 Chip via anti-His antibody can bind Human Thrombopoietin, premium grade with an affinity constant of 14.9 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 54.4 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Gln 26 - Trp 491
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MPL myeloproliferative leukemia virus oncogene [ Homo sapiens ]
• Official Symbol: MPL
• Synonyms: MPL; myeloproliferative leukemia virus oncogene; thrombopoietin receptor; CD110; TPOR; TPO-R; proto-oncogene c-Mpl; myeloproliferative leukemia protein; MPLV; C-MPL; THCYT2
• Gene ID: 4352
• mRNA Refseq: NM_005373
• Protein Refseq: NP_005364
• MIM: 159530
• UniProt ID: P40238