Active Recombinant Human/Mouse/Rat Mstn Protein (Asp268-Ser376), GMP Grade

• Cat.No.: Mstn-07HG
• Product Overview: GMP recombinant human/Mouse/Rat GDF-8/Myostatin protein (Asp268-Ser376) without tag was expressed in Mouse myeloma cell line and Manufactured and tested under current Good Manufacturing Practice (GMP) guidelines.

Specification

• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Homozygous knockout mice for this gene exhibit increased muscle mass and bone density, and reduced adiposity.
• Source: Mouse myeloma cell line
• Species: Human/Mouse/Rat
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 2-10 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 12.4 kDa
  SDS-PAGE: 12 kDa, reducing conditions; 24 kDa, non-reducing conditions
• Protein length: Asp268-Ser376
• N-terminal Sequence Analysis: Asp-Phe-Gly-Leu-Asp-(Cys)-Asp-Glu-His-Ser
• Endotoxin: <0.10 EU/ μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE with silver staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Reconstitution: Reconstitute at 100 μg/mL in 4 mM HCl.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: Mstn myostatin [ Mus musculus ]
• Official Symbol: Mstn
• Synonyms: MSTN; myostatin; growth/differentiation factor 8; GDF-8; compact; growth differentiation factor 8; Cmpt; Gdf8; MGC124261; MGC124262; MGC124263
• Gene ID: 17700
• mRNA Refseq: NM_010834
• Protein Refseq: NP_034964
• UniProt ID: O08689