Active Recombinant Human MMP14 protein, mutation C127S

• Cat.No.: MMP14-33H
• Product Overview: Recombinant Matrix Metalloproteinase-14 (MMP-14, Membrane-Type Matrix Metalloproteinase1, MT1- MMP) cloned from human cDNA, expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-14 (residues 114-290, UniProtKB accession P50281). The protein has the mutation C127S to increase its stability. The catalytic activity rates are not affected by the mutation.

Specification

• Species: Human
• Source: Non
• Protein Length: 114-290 aa
• Tag: Non
• Bio-activity: > 150 U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2- mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.
• Molecular Mass: 20.1 kDa
• AA Sequence: IQGLKWQ HNEITFSIQN YTPKVGEYAT YEAIRKAFRV WESATPLRFR EVPYAYIREG HEKQADIMIF FAEGFHGDST PFDGEGGFLA HAYFPGPNIGGDTHFDSAEP WTVRNEDLNG NDIFLVAVHE LGHALGLEHS SDPSAIMAPF
  YQWMDTENFV LPDDDRRGIQ QLYGGESGFP
• Purity: > 95% by SDS-PAGE
• Usage: Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.
• Storage: -80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: 0.2 mg/mL solution in Tris 20 mM pH 7.2, CaCl2 10 mM, ZnCl2 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.5 M.
• GeneID: 4323
• References: M. Gioia et al. J Mol Biol. 2007 May 11;368(4):1101-13.
  K. Lehti et al. J. Biol. Chem. 2000, 275:15006-13.
  G. Murphy and V. Knäuper Matrix Biol. 1997, 15, 511.
  W. Bode et al. Cell Mol Life Sci 1999, 55:639-52.

Gene Information

• Gene Name: MMP14 matrix metallopeptidase 14 [ Homo sapiens (human) ]
• Official Symbol: MMP14
• Synonyms: MMP-14,MMP-X1,MT-MMP,MT1MMP,MTMMP1,WNCHRS,MT1-MMP,MT-MMP 1,MMP14
• mRNA Refseq: NM_004995
• Protein Refseq: NP_004986
• MIM: 600754
• UniProt ID: P50281