Active Recombinant Human MKNK2 Protein, GST-tagged

• Cat.No.: MKNK2-5368H
• Product Overview: Human MKNK2 (NM_017572) full-length recombinant protein with GST tag expressed in Sf9 cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found, but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation, and in subcellular localization. [provided by RefSeq, Aug 2011]
• Bio-activity: The specific activity was determined to be 93 nmol /min/mg as per Activity Assay Protocol.
• Molecular Mass: 41 kDa
• Purity: > 70% by densitometry
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol)

Gene Information

• Gene Name: MKNK2 MAP kinase interacting serine/threonine kinase 2 [ Homo sapiens ]
• Official Symbol: MKNK2
• Synonyms: MKNK2; MAP kinase interacting serine/threonine kinase 2; G protein coupled receptor kinase 7 , GPRK7; MAP kinase-interacting serine/threonine-protein kinase 2; MNK2; Putative map kinase interacting kinase; MAPK signal-integrating kinase 2; G protein-coupled receptor kinase 7; MAP kinase signal-integrating kinase 2; GPRK7
• Gene ID: 2872
• mRNA Refseq: NM_017572
• Protein Refseq: NP_060042
• MIM: 605069
• UniProt ID: Q9HBH9