Active Recombinant Human MICB protein(Met1-Gly298), His&hFc-tagged

• Cat.No.: MICB-807H
• Product Overview: Recombinant Human MICB (NP_005922.2) (Met 1-Gly 298) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Gly298
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized human His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch, The EC50 of human MICB-Fch is 15.9-37.1 ng/ml.
• Molecular Mass: The recombinant human MICB/Fc is a disulfide-linked homodimer. The reduced monomer consists of 524 amino acids and has a predicted molecular mass of 59.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh MICB/Fc monomer is approximately 80-90 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MICB MHC class I polypeptide-related sequence B [ Homo sapiens ]
• Official Symbol: MICB
• Synonyms: MICB; MHC class I polypeptide-related sequence B; PERB11.2; MIC-B; MHC class I mic-B antigen; stress inducible class I homolog; MHC class I chain-related protein B; MHC class I-like molecule PERB11.2-IMX
• Gene ID: 4277
• mRNA Refseq: NM_005931
• Protein Refseq: NP_005922
• MIM: 602436
• UniProt ID: Q29980