Active Recombinant Human MET protein, Fc-tagged

• Cat.No.: MET-196H
• Product Overview: Recombinant Human MET protein(Glu 25 - Thr 932), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human HGF, His Tag at 2 μg/mL (100 μL/well) can bind Human HGF R, Fc Tag with a linear range of 0.6-20 ng/mL.Immobilized Human HGF R, Fc Tag at 5 μg/mL (100 μL/well) can bind Human HGF, His Tag with a linear range of 5-78 ng/mL.
• Molecular Mass: Human HGF R, Fc Tag is fused with Fc fragment of human IgG1 at the C-terminus.
  The mature form of HGF R is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 32.5 kDa (α chain) and 95.3 kDa (β chain Fc chimera). The protein migrates as 40-45 kDa (α chain) and 106-116 kDa (β chain Fc chimera) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Glu25-Thr932
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MET met proto-oncogene (hepatocyte growth factor receptor) [ Homo sapiens ]
• Official Symbol: MET
• Synonyms: MET; met proto-oncogene (hepatocyte growth factor receptor); hepatocyte growth factor receptor; HGFR; RCCP2; SF receptor; HGF receptor; oncogene MET; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase; AUTS9; c-Met
• Gene ID: 4233
• mRNA Refseq: NM_000245
• Protein Refseq: NP_000236
• MIM: 164860
• UniProt ID: P08581