Active Recombinant Human MEKK6, GST-tagged

• Cat.No.: MAP3K6-1429H
• Product Overview: Recombinant human MEKK6 (620-950) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: MEKK6 or MAP3K6 (mitogen-activated protein kinase kinase kinase 6) is a member of the serine/threonine protein kinase family. MEKK6 was identified by its interaction with MAP3K5/ASK, a protein kinase and an activator of c-Jun kinase (MAPK7/JNK) and MAPK14/p38 kinase. MEKK6 cascades relay signals from the cell surface to the nucleus which result in gene transcription. MEKK6 activates the JNK and p38 pathway and induces apoptosis when overexpressed in stably transfected cells. MEKK6 transcript is expressed in several tissues, with the highest expression in heart and skeletal muscle.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 10 nmol/min/mg
• Molecular Mass: ~67 kDa
• Protein length: 620-950 aa
• Purity: >95%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: MAP3K6 mitogen-activated protein kinase kinase kinase 6 [ Homo sapiens ]
• Official Symbol: MAP3K6
• Synonyms: MAP3K6; mitogen-activated protein kinase kinase kinase 6; apoptosis signal regulating kinase 2; ASK2; MAPKKK6; MEKK6; apoptosis signal-regulating kinase 2; MGC20114; MGC125653
• Gene ID: 9064
• mRNA Refseq: NM_004672
• Protein Refseq: NP_004663
• MIM: 604468
• UniProt ID: O95382
• Chromosome Location: 1p36.11
• Pathway: Insulin Signaling, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; p38 MAPK signaling pathway, organism-specific biosystem
• Function: ATP binding; MAP kinase kinase kinase activity; magnesium ion binding; nucleotide binding; protein serine/threonine kinase activity