Active Recombinant Human MAP1LC3B, GST-tagged

• Cat.No.: MAP1LC3B-193H
• Product Overview: Recombinant human MAP1LC3B (amino acid residues 1-120), fused with N-terminal GST, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1-120 a.a.
• Description: Three classes of enzymes are involved in autophagy; E1-like activating enzymes (E1s), E2-like conjugating enzymes (E2s) and ubiquitin-like proteins (ubls). Microtubule Associated Protein 1, Light Chain 3 beta, MAP1LC3b or LC3b is a member of the Ubl family and cloning of the human gene was first described by He et al. (2003). The C-terminal of LC3b is cleaved by ATG4B C-terminal to Gly120 in vitro and this has been shown to be required for the formation of intermediates with the ubiquitin-like activating enzyme ATG7 (Tanida et al. 2004).
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: E1 Thioester LC3b Loading Assay: The activity of LC3b was validated by loading LC3b onto the active cysteine of His-ATG7. Incubation of the His-ATG7 enzyme in the presence of LC3b and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the LC3b/His-ATG7 thioester bond to the reducing agent DTT was confirmed.
• Molecular Mass: ~41.48 kDa
• Purity: >98% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: MAP1LC3B microtubule-associated protein 1 light chain 3 beta [ Homo sapiens ]
• Official Symbol: MAP1LC3B
• Synonyms: MAP1LC3B; microtubule-associated protein 1 light chain 3 beta; microtubule-associated proteins 1A/1B light chain 3B; ATG8F; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1 light chain 3-like protein 2; autophagy-related ubiquitin-like modifier LC3 B; LC3B; MAP1LC3B-a; MAP1A/1BLC3
• Gene ID: 81631
• mRNA Refseq: NM_022818
• Protein Refseq: NP_073729
• MIM: 609604
• UniProt ID: Q9GZQ8
• Chromosome Location: 16q24.2
• Pathway: Senescence and Autophagy, organism-specific biosystem
• Function: protein binding