Active Recombinant Human Lysyl Oxidase-Like 3, His-tagged

• Cat.No.: LOXL3-1979H
• Product Overview: RecombinantHuman LOXL3 protein was expressed in Chinese HamsterOvary cell line. Met1-Ile753, with a C-terminal 10-Histag.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 1-753 a.a.
• Description: LOXL3 is asecreted copper amine oxidase. The N-terminal region of LOXL3 contains fourscavenger receptor cysteinerich (SRCR) domains. The C-terminal regionconsists of a catalytic domain similar to other lysyl oxidases and a cytokinereceptor-like domain. The catalytic domain contains conserved residues requiredfor copper binding and formation of a lysyl tyrosylquinone cofactor. LOXL3 isexpressed primarily in epidermis, kidney, aorta, and chondrocytes. The enzymeis expressed at low levels in most other tissues. Like lysyl oxidase, LOXL3oxidizes the lysine ε-amino groups of a number of collagen substrates, and isalso active against other primary amines. However, LOXL3 does not requireproteolytic processing to be activated.
• Form: Supplied as a 0.2 μmfiltered solution in MES and NaCl.
• N-terminalSequence: Ser26
• MolecularWeight: 82 kDa
• Activity: Measuredby its ability to produce hydrogen peroxide during the oxidation of benzylamine.The specific activity is > 1.5 pmoles/min/μg, as measured under the describedconditions.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 90-100kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by Colloidal CoomassieBlue stain at 5 μg per lane.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months,-20to-70°C under sterile conditions after opening.

Gene Information

• Gene Name: LOXL3 lysyl oxidase-like 3 [ Homosapiens ]
• Official Symbol: LOXL3
• Synonyms: LOXL3; lysyloxidase-like 3; LOXL; lysyl oxidase homolog 3; lysyl oxidase-likeprotein 3; EC 1.4.3.13
• Gene ID: 84695
• mRNA Refseq: NM_032603
• Protein Refseq: NP_115992
• MIM: 607163
• UniProt ID: P58215
• Chromosome Location: 2p13
• Function: copper ion binding;metal ion binding; oxidoreductase activity; oxidoreductase activity, actingon the CH-NH2 group of donors, oxygen as acceptor; protein-lysine 6-oxidaseactivity; scavenger receptor activity