Active Recombinant Human LRP5 protein, mFc-tagged

• Cat.No.: LRP5-284H
• Product Overview: Recombinant Human LRP5 protein(Glu 644 - Gln 1263), fused with mFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• Protein Length: 644-1263 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human Dkk-1, Fc,Avitag at 5 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human LRP-5, Mouse IgG2a Fc Tag with a linear range of 1-31 ng/mL.Immobilized Human Dkk-1, His Tag at 5 μg/mL (100 μL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag with a linear range of 5-156 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminus.The protein has a calculated MW of 96.6 kDa. The protein migrates as 95-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu644-Gln1263
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LRP5 low density lipoprotein receptor-related protein 5 [ Homo sapiens ]
• Official Symbol: LRP5
• Synonyms: LRP5; low density lipoprotein receptor-related protein 5; EVR1, exudative vitreoretinopathy 1 , LRP7, OPPG, osteoporosis pseudoglioma syndrome; low-density lipoprotein receptor-related protein 5; BMND1; HBM; LR3; OPS; LRP-5; low density lipoprotein receptor-related protein 7; EVR1; EVR4; LRP7; OPPG; OPTA1; VBCH2
• Gene ID: 4041
• mRNA Refseq: NM_002335
• Protein Refseq: NP_002326
• MIM: 603506
• UniProt ID: O75197