Active Recombinant Human LRP1 protein, Fc-tagged

• Cat.No.: LRP1-652H
• Product Overview: Recombinant Human LRP1 Cluster II(Arg786-Leu1165) fussed with Fc of Human IgG1 (Pro100 - Lys330) at C-terminal was expressed in NS0.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• ProteinLength: 786-1165 a.a.
• Description: LDL receptor­related protein 1 (LRP­1), also known as CD91 and the α2­macroglobulin receptor, is a type I transmembrane protein in the LDL receptor superfamily. It is expressed on neurons, hepatocytes, adipocytes, vascular smooth muscle cells, fibroblasts, keratinocytes, macrophages, and megakaryocytes. LRP­1 is important for the clearance of a large number of circulating molecules involved in fatty acid metabolism and the inhibition of serine proteases. LRP­1 also associates, or through intracellular scaffold proteins, with other membrane associated proteins on the same cell. This allows LRP­1 to modulate the activity or internalization of PDGFRβ, NMDA receptor subunits, TGF­β receptors, Frizzled­1, various integrins, and the prion proteinPrPC. Human LRP 1 is N glycosylated and sialylated, and cleaved in the Golgi to produce an 85 kDa transmembrane beta chain, and a 515 kDa alpha chain. Both associate noncovalently, with the beta chain remaining completely extracellular. The alpha chain of LRP 1 contains 31 LDLR class A repeats, 34 LDLR class B repeats, and 22 EGF­like repeats. The LDLR domains are clustered in four regions throughout the protein. Cluster II (aa 786 ­ 1165) contains one EGF­like and eight LDLR class A repeats. Cluster II contains binding sites for Apolipoprotein E, LPL, LRPAP/RAP, alpha 2 Macroglobulin, Coagulation Factor VIII light chain, Lactoferrin, PAI­1, tPA­PAI­1 complexes, Pro­uPA, and TFPI. Within this region, human LRP­1 shares 99% aa sequence identity with mouse and rat LRP­1. A shed soluble form of LRP­1 circulates in the serum and retains ligand binding properties.
• Predicted N Terminal: Arg786
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA.When rhLRP-1C2/Fc Chimera is immobilized at 50 ng/mL (100 µL/well), the concentration of rhLRPAP that produces 50% of the optimal binding response is found to be approximately 0.5 - 2.5 ng/mL.
• Molecular Mass: Predicted Molecular Mass:68.1 kDa;SDS-PAGE:90-100 kDa, reducing conditions.
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 µg/mL in PBS.

Gene Information

• Gene Name: LRP1 low density lipoprotein receptor-related protein 1 [ Homo sapiens ]
• Official Symbol: LRP1
• Synonyms: APR; LRP; A2MR; CD91; APOER; LRP1A; TGFBR5; IGFBP3R; prolow-density lipoprotein receptor-related protein 1; LRP-1; TbetaR-V/LRP-1/IGFBP-3 receptor; alpha-2-macroglobulin receptor; apolipoprotein E receptor; type V tgf-beta receptor
• Gene ID: 4035
• mRNA Refseq: NM_002332
• Protein Refseq: NP_002323
• MIM: 107770
• UniProt ID: Q07954
• Chromosome Location: 12q13.3
• Pathway: Alzheimer's disease, organism-specific biosystem; Alzheimer's disease, conserved biosystem; Binding and Uptake of Ligands by Scavenger Receptors, organism-specific biosystem
• Function: apolipoprotein binding; apolipoprotein binding; calcium ion binding