Active Recombinant Human LIMK1, GST-tagged

Description :	There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Source :	Sf9 insect cells using baculovirus
Species :	Human
Tag :	GST
Form :	Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Bio-activity :	8 nmol/min/mg
Molecular Mass :	~65 kDa
Purity :	>95%
Applications :	Kinase Assay, Western Blot
Stability :	1 yr at -70°C from date of shipment.
Storage :	Store product at –70ºC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration :	0.1ug/ul
Protein length :	285-638 a.a.

Gene Name	LIMK1 LIM domain kinase 1 [ Homo sapiens (human) ]
Official Symbol	LIMK1
Synonyms	LIMK1; LIM domain kinase 1; LIMK; LIM motif-containing protein kinase; LIMK-1; NP_001191355.1; EC 2.7.11.; NP_002305.1
Gene ID	3984
mRNA Refseq	NM_002314
Protein Refseq	NP_002305
MIM	601329
UniProt ID	P53667
Chromosome Location	7q11.23
Pathway	CDC42 signaling events; CXCR4-mediated signaling events; EPHB-mediated forward signaling; Fc gamma R-mediated phagocytosis
Function	ATP binding; heat shock protein binding; protein heterodimerization activity; protein serine/threonine kinase activity