Active Recombinant Human LILRA1 protein, His-tagged

• Cat.No.: LILRA1-267H
• Product Overview: Recombinant Human LILRA1(Pro17-Asn461) fused with His tag at C-terminal was expressed in NS0.

Specification

• Description: LILRA1, also known as CD85i and LIR-6, is an approximately 70 kDa variably glycosylated transmembrane protein that regulates immune cell activation. Mature human LILRA1 consists of a 445 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 7 aa cytoplasmic tail. The transmembrane segment contains a positively charged arginine which may mediate association with signaling molecules. Alternative splicing generates an isoform that lacks the third and fourth Ig-like domains. LILRA1 is expressed on monocytes and binds to the free heavy chain of the MHC class I molecule HLA-B27. It is activated in response to the BCG mycobacterial strain. R&D Systems in-house testing indicates that LILRA1 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Pro17 & Gly24
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human LILRA1/CD85i/LIR-6 is coated at 2 µg/mL, Recombinant Human Angiopoietin-like 7/ANGPTL7 binds with an ED50 = 0.3-1.8 µg/mL.
• Molecular Mass: Predicted Molecular Mass: 49 kDa
  SDS-PAGE: 62-76 kDa, reducing conditions
• Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Protein length: 17-461 a.a.

Gene Information

• Gene Name: LILRA1 leukocyte immunoglobulin like receptor A1 [ Homo sapiens ]
• Official Symbol: LILRA1
• Synonyms: LIR6; CD85I; LIR-6; CD85 antigen-like family member I; leucocyte Ig-like receptor A1; leukocyte immunoglobulin-like receptor 6; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1
• Gene ID: 11024
• mRNA Refseq: NM_001278318
• Protein Refseq: NP_001265247
• MIM: 604810
• UniProt ID: O75019