Active Recombinant Human LGR5 Protein, Fc Chimera

• Cat.No.: LGR5-1037H
• Product Overview: Recombinant Human LGR5 (Accession # O75473), fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Gly22
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA.When Recombinant Human R-Spondin 3 is coated at 0.5 µg/mL, Recombinant Human Lgr5/GPR49 Fc Chimera binds with an apparent Kd <0.1 nM. Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is typically 1.5-7.5 µg/mL in the presence of 20 ng/mL Recombinant Human R-Spondin 2 and 5 ng/mL Recombinant Mouse Wnt-3a.
• Molecular Mass: Recombinant Human LGR5, Fc Chimera has a calculated MW of 87 kDa (monomer). In SDS-PAGE migrates as 90-110 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: LGR5 leucine-rich repeat containing G protein-coupled receptor 5 [ Homo sapiens ]
• Official Symbol: LGR5
• Synonyms: LGR5; leucine-rich repeat containing G protein-coupled receptor 5; G protein coupled receptor 49 , GPR49, GPR67, leucine rich repeat containing G protein coupled receptor 5; leucine-rich repeat-containing G-protein coupled receptor 5; FEX; HG38; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; orphan G protein-coupled receptor HG38; leucine-rich repeat-containing G protein-coupled receptor 5; GPR49; GPR67; GRP49; MGC117008
• Gene ID: 8549
• mRNA Refseq: NM_003667
• Protein Refseq: NP_003658
• MIM: 606667
• UniProt ID: O75473
• Chromosome Location: 12q22-q23