Active Recombinant Human LGALS8 Protein (Met2-Trp317), N-His tagged, Animal-free, Carrier-free

• Cat.No.: LGALS8-194H
• Product Overview: Recombinant Human LGALS8 Protein (Met2-Trp317) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Galectin-8 (Gal-8) is a lectin family member and is one of the tandem repeat-type galectins containing two carbohydrate recognition domains (CRD) connected by a linker region in a single peptide chain. The CRD is responsible for β-galactoside binding, and the binding partners for galectin-8 have been identified, including CD44 and a subset of integrins. Galectin-8 and integrins form complexes, subsequently leading to phosphorylation of paxillin and focal adhesion kinase (FAK), which activates signal pathways including MAPK-ERK and PI3K-AKT pathways. Galectin-8 is constitutively presented in the Liver, kidney, cardiac muscle, lung, and brain. Galectin-8 serves as an immunosuppressive protective role against autoimmune CNS inflammation, regulating the balance of Th17 and Th1 polarization.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is < 8 μg/mL.
• Molecular Mass: The protein has a calculated MW of 36.6 kDa.
  The protein migrates as 34 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Met2-Trp317
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: LGALS8 lectin, galactoside-binding, soluble, 8 [ Homo sapiens (human) ]
• Official Symbol: LGALS8
• Synonyms: LGALS8; lectin, galactoside-binding, soluble, 8; galectin-8; galectin 8; PCTA 1; galectin-8g; Po66 carbohydrate binding protein; po66 carbohydrate-binding protein; prostate carcinoma tumor antigen 1; Gal-8; PCTA1; PCTA-1; Po66-CBP
• Gene ID: 3964
• mRNA Refseq: NM_006499
• Protein Refseq: NP_006490
• MIM: 606099
• UniProt ID: O00214