Active Recombinant Human LGALS4 protein, His-tagged

• Cat.No.: LGALS4-10H
• Product Overview: Active Recombinant Human LGALS4 protein(Met1~Ile323), fused with N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: Met1~Ile323
• Form: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Bio-activity: Galectin-4(GAL4) is a protein that in humans is encoded by the LGALS4 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS4 is an S-type lectin that is strongly underexpressed in colorectal cancer. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant human GAL4 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL4 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL4, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The minimal effective concentration of GAL4 is 3.187μg/mL.
• Molecular Mass: 37.2kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method)
• Purity: > 92%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: LGALS4 lectin, galactoside-binding, soluble, 4 [ Homo sapiens ]
• Official Symbol: LGALS4
• Synonyms: LGALS4; lectin, galactoside-binding, soluble, 4; galectin-4; GAL4; galectin 4; gal-4; antigen NY-CO-27; lactose-binding lectin 4; L-36 lactose-binding protein; L36LBP
• Gene ID: 3960
• mRNA Refseq: NM_006149
• Protein Refseq: NP_006140
• MIM: 602518
• UniProt ID: P56470