Active Recombinant Human Leptin Antagonist Triple Mutant, PEG-tagged

Cat.No. : LEP-260H
Product Overview : Pegylated Leptin Antagonist Triple Mutant Human Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular weight of 35.6kDa, Leptin was mutated, resulting in L39A/D40A/F41A. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 48 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. Leptin Antagonist Triple Mutant Human Recombinant is Mono-Pegylated with 20kDa PEG and was purified by proprietary chromatographic techniques.
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Species : Human
Source : Human
Tag : Non
Form : The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
Purity : Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
Bio-activity : Capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is 6-8 fold lower than the non-pegylated antagonist but in vivo it has profound weight gain effect in mice (as compared to the non-pegylated antagonist), resulting mainly from increased food intake.
Physical Appearance : White lyophilized (freeze-dried) powder.
Solubility : It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant pegylated Human Recombinant in sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Protein content : Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Storage : Lyophilized Pegylated Leptin Antagonist Triple Mutant Human Recombinant although stable at room temperature for several weeks, should be C. Upon reconstitution at°stored desiccated below -18 > 0.1 mg/ml and up to C or even room 2mM and filter sterilization Leptin mutant can be stored at 4 temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Gene Name LEP leptin [ Homo sapiens ]
Official Symbol LEP
Synonyms LEP; leptin; OB; OBS; FLJ94114; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Obese protein; Obesity factor
Gene ID 3952
mRNA Refseq NM_000230
Protein Refseq NP_000221
MIM 164160
UniProt ID P41159
Chromosome Location 7q31.3
Pathway Adipocytokine signaling pathway; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Neuroactive ligand-receptor interaction
Function growth factor activity; hormone activity; peptide hormone receptor binding; protein binding

Not For Human Consumption!

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