Active Recombinant Human Leptin Antagonist Triple Mutant, PEG-tagged
Cat.No. : | LEP-260H |
Product Overview : | Pegylated Leptin Antagonist Triple Mutant Human Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular weight of 35.6kDa, Leptin was mutated, resulting in L39A/D40A/F41A. However due to enlarged hydrodymanic volume it runs on the SDS-PAGE as 48 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. Leptin Antagonist Triple Mutant Human Recombinant is Mono-Pegylated with 20kDa PEG and was purified by proprietary chromatographic techniques. |
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Species : | Human |
Source : | Human |
Tag : | Non |
Form : | The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3. |
Purity : | Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE. |
Bio-activity : | Capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is 6-8 fold lower than the non-pegylated antagonist but in vivo it has profound weight gain effect in mice (as compared to the non-pegylated antagonist), resulting mainly from increased food intake. |
Physical Appearance : | White lyophilized (freeze-dried) powder. |
Solubility : | It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant pegylated Human Recombinant in sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein content : | Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.88 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
Storage : | Lyophilized Pegylated Leptin Antagonist Triple Mutant Human Recombinant although stable at room temperature for several weeks, should be C. Upon reconstitution at°stored desiccated below -18 > 0.1 mg/ml and up to C or even room 2mM and filter sterilization Leptin mutant can be stored at 4 temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles. |
Gene Name | LEP leptin [ Homo sapiens ] |
Official Symbol | LEP |
Synonyms | LEP; leptin; OB; OBS; FLJ94114; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Obese protein; Obesity factor |
Gene ID | 3952 |
mRNA Refseq | NM_000230 |
Protein Refseq | NP_000221 |
MIM | 164160 |
UniProt ID | P41159 |
Chromosome Location | 7q31.3 |
Pathway | Adipocytokine signaling pathway; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Neuroactive ligand-receptor interaction |
Function | growth factor activity; hormone activity; peptide hormone receptor binding; protein binding |
◆ Recombinant Proteins | ||
Lep-146M | Recombinant Mouse Leptin, PEG | +Inquiry |
LEP-260H | Active Recombinant Human Leptin Antagonist Triple Mutant, PEG-tagged | +Inquiry |
LEP-1085D | Recombinant Dog LEP Protein, His-tagged | +Inquiry |
Lep-226M | Recombinant Mouse Lep | +Inquiry |
LEP-3032R | Recombinant Rat LEP Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Native Proteins | ||
LEP-27641TH | Native Human LEP | +Inquiry |
◆ Cell & Tissue Lysates | ||
LEP-4773HCL | Recombinant Human LEP 293 Cell Lysate | +Inquiry |
Not For Human Consumption!
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