Acitve Recombinant Human LEP protein(Val22-Cys167)

• Cat.No.: LEP-1272H
• Product Overview: Recombinant Human Leptin (NP_000221.1) (Val 22-Cys 167) was expressed in E. coli, with an additional Met.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: Val22-Cys167
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Human Leptin at 2 μg/mL (100 μL/well) can bind Human Leptin Receptor (His & hFc Tag), the EC50 of Human Leptin Receptor (His & hFc Tag) is 70-420 ng/mL.
• Molecular Mass: The recombinant Human leptin consists of 147 amino acids and predicts a molecular mass of 16 kDa. The apparent molecular mass of rhLEP is approximately 13 kDa in SDS-PAGE under reducing conditions.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LEP leptin [ Homo sapiens ]
• Official Symbol: LEP
• Synonyms: LEP; leptin; leptin (murine obesity homolog) , leptin (obesity homolog, mouse) , OB, OBS; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); OB; OBS; FLJ94114
• Gene ID: 3952
• mRNA Refseq: NM_000230
• Protein Refseq: NP_000221
• UniProt ID: P41159