Active Recombinant Full Length Human LBP Protein

• Cat.No.: LBP-10HFL
• Product Overview: Recombinant Full Length Human LBP Protein without tag was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: NS0
• Tag: Non
• Protein Length: Full Length
• Description: The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP).
• Bio-activity: Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 0.25-2.5 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 51 kDa
  SDS-PAGE: 55-60 kDa, reducing conditions
• N-terminal Sequence Analysis: Ala26
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and EDTA with BSA as a carrier protein.
• Reconstitution: Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: LBP lipopolysaccharide binding protein [ Homo sapiens (human) ]
• Official Symbol: LBP
• Synonyms: LBP; lipopolysaccharide binding protein; BPIFD2; lipopolysaccharide-binding protein; BPI fold containing family D, member 2; LPS-binding protein
• Gene ID: 3929
• mRNA Refseq: NM_004139
• Protein Refseq: NP_004130
• MIM: 151990
• UniProt ID: P18428