Active Recombinant Human LAG3 Protein, mFc-tagged

• Cat.No.: LAG3-383H
• Product Overview: Recombinant Human LAG3 Protein, MIgG2a Fc-tagged is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1). Predicted N-terminus: Leu 23. This protein carries a mouse IgG2a Fc tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• Protein Length: Leu 23 - Leu 450
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human LAG-3, Mouse IgG2a Fc Tag at 2 μg/mL (100 μL/well) can bind Anti-Human LAG3 MAb, Human IgG1 with a linear range of 1-10 ng/mL.
• Molecular Mass: The protein has a calculated MW of 73.3 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
  >95% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: LAG3 lymphocyte-activation gene 3 [ Homo sapiens ]
• Official Symbol: LAG3
• Synonyms: LAG3; lymphocyte-activation gene 3; lymphocyte activation gene 3 protein; CD223
• Gene ID: 3902
• mRNA Refseq: NM_002286
• Protein Refseq: NP_002277
• MIM: 153337
• UniProt ID: P18627