Active Recombinant Human LAG3 protein, His-tagged

• Cat.No.: LAG3-104H
• Product Overview: Recombinant Human LAG3 protein(Leu 23 - Leu 450), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 23-450 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Anti-Human LAG3 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Human LAG-3 Protein, His Tag with a linear range of 1-6 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 47.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu23-Leu450
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LAG3 lymphocyte-activation gene 3 [ Homo sapiens ]
• Official Symbol: LAG3
• Synonyms: LAG3; lymphocyte-activation gene 3; lymphocyte activation gene 3 protein; CD223
• Gene ID: 3902
• mRNA Refseq: NM_002286
• Protein Refseq: NP_002277
• MIM: 153337
• UniProt ID: P18627