Active Recombinant Human KMO Protein, N-6×His tagged

• Cat.No.: KMO-07H
• Product Overview: Recombinant Human KMO Protein (Asp2-Leu441) with an N-terminal Met and 6×His tag was expressed in Spodoptera frugiperda, Sf 21 (Insect cells).

Specification

• Description: This gene encodes a mitochondrion outer membrane protein that catalyzes the hydroxylation of L-tryptophan metabolite, L-kynurenine, to form L-3-hydroxykynurenine. Studies in yeast identified this gene as a therapeutic target for Huntington disease.
• Source: Insect cells
• Species: Human
• Tag: His
• Bio-activity: Measured by the consumption of NADPH by hydroxylation of L-kynurenine to 3-hydroxy-kynurenine. The specific activity is > 290 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 51 kDa
  SDS-PAGE: 40-45 kDa, reducing conditions
• N-terminal Sequence Analysis: Inconclusive results, intact N-terminus verified by anti-poly-His Western
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: > 80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
• Shipping: The product is shipped with dry ice or equivalent.
• Protein length: 2-441 a.a.

Gene Information

• Gene Name: KMO kynurenine 3-monooxygenase (kynurenine 3-hydroxylase) [ Homo sapiens (human) ]
• Official Symbol: KMO
• Synonyms: KMO; kynurenine 3-monooxygenase (kynurenine 3-hydroxylase); kynurenine 3-monooxygenase; kynurenine 3-hydroxylase; dJ317G22.1
• Gene ID: 8564
• mRNA Refseq: NM_003679
• Protein Refseq: NP_003670
• MIM: 603538
• UniProt ID: O15229