Active Recombinant Human KLRK1 protein, lFc-tagged

Cat.No. : KLRK1-0580H
Product Overview : Recombinant Human KLRK1 protein(Ile73-Val216), fused with lFc tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : lFc
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human MICA, His Tag at 5 μg/mL (100 μL/well) can bind Human NKG2D, Llama IgG2b Fc Tag with a linear range of 0.01-0.625 μg/mL. Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
Molecular Mass : This protein carries a llama IgG2b Fc tag at the N-terminus.The protein has a calculated MW of 44.5 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Proteinlength : Ile73-Val216
Endotoxin : Less than 0.01 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Protein length : 73-216 a.a.
Gene Name KLRK1 killer cell lectin-like receptor subfamily K, member 1 [ Homo sapiens ]
Official Symbol KLRK1
Synonyms KLRK1; killer cell lectin-like receptor subfamily K, member 1; D12S2489E, DNA segment on chromosome 12 (unique) 2489 expressed sequence; NKG2-D type II integral membrane protein; CD314; KLR; NKG2 D; NKG2D; NK cell receptor D; NKG2-D-activating NK receptor; NKG2-D; D12S2489E; FLJ17759; FLJ75772;
Gene ID 22914
mRNA Refseq NM_007360
Protein Refseq NP_031386
MIM 611817
UniProt ID P26718

Not For Human Consumption!

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