Active Recombinant Human Klotho Beta, His-tagged

• Cat.No.: KLB-5671H
• Product Overview: Recombinant Human KLBprotein was expressed in Murine myeloma cell line with a C-terminal 10-Histag. Phe53-Leu997.

Specification

• Cat. No.: KLB-5671H
• Description: KLB, a divergentstructural member of the glycosidase I superfamily, is expressed primarily inthe liver and pancreas, with lower expression in adipose tissue. Like Klotho,KLB facilitates binding between FGF19 subfamily members and their receptorsvia formation of a ternary complex. The KLB mediated interaction of humanFGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulatesbile acid synthesis by controlling the secretion of two key bile acidsynthase genes, Cyp7a1 and Cyp8b1.
• Source: Mammalian cells
• Form: Supplied as a 0.2 μmfiltered solution in PBS, Glycerol and EDTA.
• N-terminalSequence: Phe53
• Molecular Weight: 110 kDa
• Activity: Measuredby its binding ability in a functional ELISA. When 2 μg/mL (100 μL/well) ofrhKLB was immobilized onto Ms x hKLB coated plate, the concentration ofrhFGF-21 that produces 50% of the optimal binding response was found to beapproximately 3-18 ng/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 126 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after opening.
• Protein length: 53-997 a.a.

Gene Information

• Gene Name: KLB klotho beta [ Homosapiens ]
• Official Symbol: KLB
• Synonyms: KLB; klotho beta;BKL; MGC142213; beta-klotho; betaKlotho; klotho beta like; klotho beta-likeprotein; EC 3.2.18; OTTHUMP00000158728
• Gene ID: 152831
• mRNA Refseq: NM_175737
• Protein Refseq: NP_783864
• MIM: 611135
• UniProt ID: Q86Z14
• Chromosome Location: 4p14
• Pathway: Downstream signalingof activated FGFR; FGF signaling pat; FGFR ligand binding and activation;FRS2-mediated cascade; IRS-mediated signalling; IRS-related events; Insulinreceptor signalling cascade; Klotho-mediated ligand binding; Negativeregulation of FGFR signaling; PI-3K cascade; PI3K Cascade; PhospholipaseC-mediated cascade; SHC-mediated cascade; Signal Transduction; Signaling byFGFR
• Function: catalytic activity;cation binding; fibroblast growth factor binding; hydrolase activity