Active Recombinant Human KLK2 protein, His-tagged

• Cat.No.: KLK2-152H
• Product Overview: Recombinant Human KLK2(Met1-Pro261) fused with His tag at C-terminal was expressed in NS0.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 1-261 a.a.
• Description: Kallikrein 2 (KLK2) is a secreted serine protease that is highly expressed in the human prostate gland. It is also known as the prostate-specific glandular kallikrein. The enzyme is highly specific for cleavage after arginine residues. KLK2 is able to activate the urokinase-type plasminogen activator. KLK2 is inhibited by serpins such as protein C inhibitor, antichymotrypsin, and plasminogen activator inhibitor 1. KLK2 is structurally related to KLK3, the prostate-specific antigen (PSA). Like PSA, KLK2 is considered to be a biomarker for prostate cancer.
• Predicted N Terminal: Ile25 & Leu124
• Form: Supplied as a 0.2 μm filtered solution in Sodium Citrate and NaCl.
• Bio-activity: Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >125 pmol/min/μg.
• Molecular Mass: Predicted Molecular Mass: 28 & 16 kDa;SDS-PAGE: 34 kDa & 15-18 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 centigrade as supplied.3 months, -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: KLK2 kallikrein-related peptidase 2 [ Homo sapiens ]
• Official Symbol: KLK2
• Synonyms: KLK2; kallikrein-related peptidase 2; kallikrein 2, prostatic; kallikrein-2; tissue kallikrein-2; glandular kallikrein 2; glandular kallikrein-1; hK2; hGK-1; KLK2A2; FLJ17010; FLJ17011; MGC12201
• Gene ID: 3817
• mRNA Refseq: NM_001002231
• Protein Refseq: NP_001002231
• MIM: 147960
• UniProt ID: P20151
• Chromosome Location: 19q13.33
• Pathway: Activation of Matrix Metalloproteinases, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, conserved biosystem; Extracellular matrix organization, organism-specific biosystem
• Function: peptidase activity; serine-type endopeptidase activity